Mikroskop digunakan dalam kaedah penyelidikan mikrobiologi. Mikroskop berfungsi untuk membesarkan imej dengan menggunakan dua sistem kanta: Kanta objektif yang paling dekat dengan spesimen dan kanta mata atau kanta okular yang terletak dibahagian atas mikroskop.
Kanta objektif membesarkan spesimen untuk memberi imej sebenar yang disalurkan keatas melalui satah fokus kanta okular. Imej itu akan kemudiannya dibesarkan oleh kanta okular untuk memberi imej maya yang dilihat oleh mata.
Mikroskop berjenama Olympus CX mempunyai empat kanta objektif yang mempunyai kuasa pembesaran 4x, 10x, 40xdan 100x rendaman minyak.
Kanta objektif 4x adalah terpendek dan mempunyai jarak berkerja (jarak antara kanta dan slaid) sejauh 22mm sementara kanta objektif 10x, 40x dan 100x rendaman minyak mempunyai jarak berkerja 10.5, 0.56 dan 0.133 mm setiap satu.
2.0 LAB PRACTICAL 1: ISOLATION AND IDENTIFICATION OF MICROBES
Media Treatment
INTRODUCTION
In microbiology study, it is important to study the mode and route of infection of microbes that causes disease to the host plants or animals. The microbes that cause diseases oftenly be known to be pathogenic. The microbes that cause disease can be found anywhere. In such, this experiment was conducted to identify the microbes that can be found from everywhere that near to us.
OBJECTIVE
- To identify the microbes found in our cough, touh, water, air and naturally exist within us.
PROCEDURE
5 set of petri dish were prepared, with every set contain 2 NA petri dish and 2 PDA petri dish:
1. I set of petri dish was left as control. DO NOT OPEN THE PETRI DISH.
2. 1 set of petri dish was opened for 2 minutes and it was closed back.
3. 1 set of petri dish was touched with finger (more or less 2-3 touch) and it was closed back.
4. 1 set of petri dish was coughed and it was closed back.
5. 1 set of petri dish was smeared with one drop of water using the steriled inoculation loop.
RESULT
Petri dish culture of PDA and NA was observed and checked for the colonies exist.
1. Control (The unopened petri dish).
PDA (potato dextrose agar)
NA (nutrient agar)
PDA (potato dexrose agar)
4. Coughed Petri Dish
NA (nutrient agar)
PDA (potato dextrose agar)
5. Smeared water on petri dish
NA (nutrient agar)
PDA (potatoe dextrose agar)
DISCUSSION
From the experiment, we found out that microbes exist and living within us. In the petri dish that was used as control that never opened, there were none of the microbes that identified on the petri dish. From hre we can infer that the pteri dish and the media agar were really free from any microbes that may cause infections. However, on an opened petri dish of both media, there is presence of microbial livinf on the petri dish. From here, we can infer that the air also contain microbes that can be sporadically spread and cause infection to the media.
Moreover, it was also found out that microbes eventually exist in the esophagus tract of human. This claimed was made due to we found out that there were signs of microbes infection on the petri dish coughed by students. Hence, microbes such as bacteria definitely can stay inside our body and causes infection and later make us human to fall sick. Not only that, we also found out that there were microbes on the plates smeared by tap water from the laboratory sink. This conclude that, even water that used daily have risk of microbes if not being boiled before used.
Lastly, we also found out that microbes such as Aspergillus sp. , bacteria and fungus was identified on our palm as the petri dish that was touch by one of the group member was grown with fungus. From there, we knew that the fungus spores can be easily spread by human touch.
CONCLUSION
From the study, we can conclude that everyday we, human were in risk of infection by variety of microbes from surrounding us. Hence, we are in-need to take proper measure and care towards the water we consumed and activities we are doing so that we stay healthy and free from risk of infection.
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